Short Courses

Diffraction-limited resolution had prevented resolving details smaller than half the wavelength of light (~200 nm) by optical microscopy. Since 1994, diffraction barrier has been overcome by several approaches in fluorescence microscopy including stimulated emission depletion (STED) microscopy, structured illumination microscopy (SIM), single-molecule localization microscopy (SMLM), etc. Currently they are widely used in diverse areas and its concepts has been extended beyond fluorescence microscopy. This course introduces the basic principles and features of super-resolution microscopy and its current applications to optics, physics, chemistry, materials science, biology and medicine. Further discussion will be made including deep learning based super-resolution imaging.

This course should enable participants to …

• Define diffraction limit in optical imaging system
• Determine and measure spatial resolution in optical microscopy
• Explain basic working principles of nanoscopy techniques including STED, SIM, SMLM
• Calculate and predict spatial resolution in nanoscopy
• Describe experimental schemes and imaging analysis
• List optical components including laser, fiber, SLM, etc.  
• Compare pros and cons of nanoscopy with those of conventional techniques
• Discuss future prospects of nanoscopy in diverse areas

This course does not require prior knowledge.

Dr. Kyu Young Han is an associate professor at CREOL, The College of Optics and Photonics, University of Central Florida and he has been developing next generation super-resolution microscopy, high-throughput imaging and quantitative single-molecule imaging tools. During his PhD, he worked at Max Planck Institute for Biophysical Chemistry in Germany where he studied STED microscopy. He has a patent on STED microscopy that was licensed to Leica and Abberior Instruments. He is a recipient of the NIH MIRA Early Stage Investigators.